Using a Bioinformatics Approach to Search Splice Sites Regulated by RNA editing

Abstract

RNA editing is nucleotide conversion which occurred by the emzyme which the adenosine deaminases that act on RNA (ADAR). RNA editing is a post-transcription processing, the occurred conditions are the sequences formed the double-stranded RNA, and the nucleotide is conversed by ADAR emzyme, the nucleotide conversion is adonsine (A) to inosine (I). In recently studies, some genes have alternative splicing events regulated by RNA editing. We used a bioinformatics approach to search splice sites regulated by RNA editing and searched and analyzed for three species: human, rat, and mouse. Total 23 mRNA sequences that occurred RNA editing were found out, and 21 editing positions are different. Then also search alternative splicing regulated by RNA editing, trinucleotide analysis, and cross-species comparison.