Improvement and construction of RNA Silencing Model with hybrid functional Petri net

Abstract

RNA interference, also known as RNAi, RNA silencing, or posttranscriptional gene silencing (PTGS) is a mechanism presented in almost all eukaryotes that functions like miniature immune system. The mechanism of RNA interference is a successive phenomenon taken place from DNA level to protein level. In this paper, we have modified the RNA silencing mechanism by adding four important factors that did not show in other works. Those factors are stochastic translocation of mRNA, stochastic dsRNA synthesis, formation of RISC protein, and randomly functions of primed as well as unprimed dsRNA supply. We also construct our new model using Hybrid functional Petri net. The simulation results of our model show that our findings are strong consistent with known biological phenomena.